A novel procedure for absolute real-time quantification of gene expression patterns

Table 2 Quantitation of absolute numbers of bh2c transcript copies in the corolla samples of Ipomoea purpurea

Sample name	Type	Ct	Given concentration (copies/μl)	Calculated Concentration (copies/μl)	cDNAs concentration* (pg/μl)	Transcript copies/fg cDNAs**
bh2c-1	Standard	6.67	117363.39	128533.38
bh2c-2	Standard	10.21	11736.33	10758.19
bh2c-3	Standard	13.24	1173.63	1284.77
bh2c-4	Standard	16.83	117.36	104.04
bh2c-5	Standard	19.93	11.74	11.80
bh2c-6	Standard	23.4	1.17	1.04
bh2c-7	Standard	26.31	0.117	0.135
bh2c 2008-9-23	Unknown	13.22 ± 0.03		1302.40 ± 24.43	9101.52	143.10 ± 2.68
bh2c 2008-9-24	Unknown	20.02 ± 0.04		11.14 ± 0.30	10285.83	1.08 ± 0.03
bh2c 2008-9-25	Unknown	14.08 ± 0.02		714.67 ± 8.61	10582.80	67.53 ± 0.81
bh2c 2008-9-26	Unknown	13.95 ± 0.01		784.27 ± 6.37	6614.55	118.57 ± 0.96
bh2c 2008-9-27	Unknown	13.27 ± 0.01		1261.69 ± 5.28	10534.68	119.77 ± 0.50

*The concentrations of cDNAs (pg/μl) were derived from Table 1 after taking 50 × factor into account.
** The copy number was estimated via equation (1). Standard errors (± se) are based on triplet measurements.

ISSN: 1746-4811