Figure 3
From: A novel procedure for absolute real-time quantification of gene expression patterns
Expression patterns of target genes during corolla development of Ipomoea purpurea. Three anthocyanin genes were compared. IpF3'H (accession number: EU032626) and IpWDR1 (accession number: EU032621) were both from genotype GZKL, and IpDFR-B (accession number: AB018438) was from III6D. (A) Expression pattern after normalized by the geometric means of the reference gene transcript numbers. (B) Direct estimates of transcript copy numbers by the CBA method, without normalization. (C) By the relative quantification method, with reference genes IpACTIN4 and IpGAPDH2 expressed in the same samples and calibrated at the time of flowering (t = 0).