Figure 5
From: Isolation of dimorphic chloroplasts from the single-cell C4 species Bienertia sinuspersici
Formation of calcium oxalate crystals in the vacuole-derived vesicles. The vesicles were obtained from lysis of protoplasts by osmotic swelling at 0.2 M sucrose and isolated on the floating layer after centrifugation at 100g, 4 min. Formation of calcium oxalate crystals was induced by resuspension of the vesicles in cell stabilizing buffer with 0.7 M sucrose. (A) A light micrograph of prismatic crystals. (B) A light micrograph of raphide crystals. (C) A light micrograph of both prismatic and raphide crystals. Scale bars = 20 μm.