Figure 4
From: Isolation of dimorphic chloroplasts from the single-cell C4 species Bienertia sinuspersici
Vacuolar staining of chlorenchyma protoplasts and osmotically-derived vesicles. Isolated protoplasts were stained with 5-(and-6)-carboxy-2',7'-dichloro-fluorescein diacetate (CDCFDA) and lysed by osmotic swelling. The isolated protoplasts and induced vesicles were observed under light and epifluorescence microscopy. CDCFDA staining, chlorophyll autofluorescence, merged images of the two channels, and bright field images are shown. (A) An isolated protoplast. (B) A swelling protoplast before cell lysis. (C) A vacuole-derived vesicle. Scale bars = 10 μm.