Figure 3
From: Isolation of dimorphic chloroplasts from the single-cell C4 species Bienertia sinuspersici
Time-lapse imaging of protoplasts subjected to osmotic swelling. (A) Diluting the cell stabilizing buffer to a sucrose concentration of 0.1 M led to rapid swelling and bursting of both the protoplasts and their vacuoles. (B) Lowering the osmotic potential of the cell stabilizing buffer to the optimum 0.2 M sucrose resulted in protoplast lysis and the release of intact central cytoplasmic compartments and vacuoles with attached peripheral chloroplasts. (C) Subtle dilution of the cell stabilizing buffer to 0.5 M sucrose did not lyse the protoplasts while vesicles were slowly pinching off from the vacuoles of protoplasts as indicated by arrowheads. Scale bars = 20 μm.