Figure 1
From: Isolation of dimorphic chloroplasts from the single-cell C4 species Bienertia sinuspersici
Illustration of the cellular and subcellular morphologies during the process of dimorphic chloroplast isolation. (A) A mature chlorenchyma cell. For clarity, only one cytoplasmic channel is illustrated despite the multiple occurrences in an actual cell. (B) An isolated protoplast with digested cell wall. (C) A swelling protoplast with increased osmotic pressure of the cell sap as indicated by arrows. (D) A broken protoplast with torn plasma membrane as indicated by arrows. (E) An intact vacuole carrying peripheral chloroplasts on the external surface. (F) An isolated central cytoplasmic compartment. Scale bars = 10 μm (A-E) or 5 μm (F).