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Figure 4 | Plant Methods

Figure 4

From: A rice transient assay system identifies a novel domain in NRR required for interaction with NH1/OsNPR1 and inhibition of NH1-mediated transcriptional activation

Figure 4

Effects of the point mutations on the ability of NRR to repress NH1-mediated activation. (A) Protoplast transient assay. The experiment was done as in Figure 1. (B) Western blot analysis of total protein extracted from protoplast cells transfected with the different combinations of plasmids. Four replicates of transfection for each combination of plasmids were carried out and the cells combined at the end of transfection. Cells were incubated for 20 h before harvest. The amount of protein loaded for each sample was normalized to the Gus activity expressed from the Ubi-Gus construct included as a reference. Protein on the blot was probed with an anti-His antibody. The wild type NRR protein and mutants W66, WF, and DL are tagged with 6× histidine at the C-terminus.

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Plant Methods

ISSN: 1746-4811