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Figure 2 | Plant Methods

Figure 2

From: A rice transient assay system identifies a novel domain in NRR required for interaction with NH1/OsNPR1 and inhibition of NH1-mediated transcriptional activation

Figure 2

Transient assay for effects of NRR, RH1, RH2, and RH3 on NH1-mediated activation. (A) Protoplasts preparation and transfection were done as described in Figure 1. The Gal4:rLG2 and Ubi-NH1 constructs are as described above. NRR, RH1, RH2, and RH3 were expressed from the Ubi-1 promoter. Each bar represents the average and standard deviation of three replicates. (B) Sequence lineup of the NPR1-interacting domain and the NH1-interacting domain. Sequences within the NPR1 interacting and NH1-interacting domains of rice NRR, RH1, RH2, RH3, and wheat NRR ortholog (wNRR) are lined up. A consensus sequence is deduced. (C) Yeast two-hybrid assay. The NH1 protein is fused to the LexA protein (bait) and NRR, RH1, RH2, and RH3 are fused to the B42AD protein (prey). A positive interaction between the bait and the prey results in blue colors.

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Plant Methods

ISSN: 1746-4811