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Figure 1 | Plant Methods

Figure 1

From: A rice transient assay system identifies a novel domain in NRR required for interaction with NH1/OsNPR1 and inhibition of NH1-mediated transcriptional activation

Figure 1

Transient cell assay on transcriptional activation activity of rTGA2.1, rLG2, and NH1. Protoplast cells were prepared from 10 days old green, transgenic rice seedlings, containing a UAS-Luc reporter. Protoplasts were transfected with combinations of plasmid constructs and Luc and Gus enzyme activities assayed after 20 h incubation at 28°C in growth chamber. The Ubi-Gus plasmid was included in all transfections and the Gus activity assayed for reference. In blanks, a Ubi-pUC plasmid was included to compensate for the amount of input DNA. rTGA2.1 and rLG2 are fused to the Gal4 DNA binding domain respectively, generating Gal4:rTGA2.1 and Gal4:rLG2. NH1 was expressed from the Ubi-NH1 construct. The UAS-Luc reporter activity is expressed as Luc/Gus. Each bar represents the average and standard deviation of three independent transfections.

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Plant Methods

ISSN: 1746-4811