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Figure 2 | Plant Methods

Figure 2

From: CalloseMeasurer: a novel software solution to measure callose deposition and recognise spreading callose patterns

Figure 2

The CalloseMeasurer analysis workflow for measuring callose deposits. (A) The reconstructed ROI objects (Figure 2F) are divided into two groups – a “big” callose group (A) and a “small” callose group (D), both are randomly coloured. (B, C) In the “big” callose group, the border of callose is recalculated (callose objects are merged/separated based on the callose centres shown in Figure 2D). Some wrongly recognised callose candidates are removed. (D) Similar to the “big” callose group, the border of every small callose is measured and some wrongly detected ones are removed. (E, F) The refined “small” and “big” callose groups are merged (coloured red) and treated as callose deposition objects (randomly coloured). Quantifiable results (e.g., size, circularity, and intensity) are exported to two CSV files – one for every processed image and one for overall results.

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Plant Methods

ISSN: 1746-4811