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Figure 3 | Plant Methods

Figure 3

From: Protocol: Chromatin immunoprecipitation (ChIP) methodology to investigate histone modifications in two model diatom species

Figure 3

Analysis of antibody specificity using peptide competition assays on western blots of P. tricornutum nuclear extracts. (A ) Upper lane contains from left to right 1 μg of H3K4me2 antibody alone or with 0.25 μg of one of the different modified peptides, H3K4me1, H3K4me2 and H3K4me3. Lower lane contains H4 antibody as internal loading control. (B) Upper lane contains 1 μg of H3K9me2 antibody alone or with 0.25 μg of one of the different modified peptides, H3K9me1, H3K9me2 and H3K9me3. Lower lane contains H4 antibody as internal loading control.

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Plant Methods

ISSN: 1746-4811