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Figure 2 | Plant Methods

Figure 2

From: Protocol: Chromatin immunoprecipitation (ChIP) methodology to investigate histone modifications in two model diatom species

Figure 2

Sonication efficiency. Different times and number of cycles were used for sonication. After crosslinking, eluted DNA was loaded in a 2% gel to monitor the efficiency of sonication. From left to right, ladder, 10 times diluted sample containing 500000 cells/ml with 7 cycles 25 seconds ON and 1 minute OFF, original sample from 200 ml culture containing 5 million cells/ml, 4x and 2x diluted samples with 7 cycles, 25 seconds and 1 minute OFF and 4x diluted sample with 9 cycles, 30 seconds ON and 1 minute OFF.

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Plant Methods

ISSN: 1746-4811