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Table 2 Recovery of phytohormones from different sample matrices on polystyrene/ divinylbenzene-based Cromabond® HR-XC material

From: An UPLC-MS/MS method for highly sensitive high-throughput analysis of phytohormones in plant tissues

Matrix SA ABA JA 12-OH-JA JA-Ile OPDA
  Slope R 2 Rec. Slope R 2 Rec. Slope R 2 Rec. Slope R 2 Rec. Slope R 2 Rec. Slope R 2 Rec.
Recovery control 453.48 0.99 1.00 75.12 0.99 1.00 105.27 0.99 1.00 431.91 0.99 1.00 541.36 0.99 1.00 126.64 0.99 1.00
20% Methanol 397.59 0.99 0.88 90.73 0.99 1.21 110.37 0.97 1.05 497.68 1.00 1.15 533.24 1.00 0.98 122.33 1.00 0.97
Methanolic extract 558.56 0.96 1.23 76.50 0.92 1.02 108.49 0.97 1.03 444.99 0.98 1.03 532.82 1.00 0.98 118.41 0.99 0.93
Reconstituted methanolic extract 392.81 0.77 0.86 55.25 0.86 0.49 81.27 0.88 0.64 156.24 0.83 0.44 424.25 0.99 0.67 77.65 0.95 0.50
  1. The analyte abundances (peak heights) were plotted against their defined concentrations and correlation of these two parameters was estimated (linear regression coefficient was calculated). The phytohormone recoveries (Rec.) were determined as ratios of slopes compairing corresponding concentration-intensity plots obtained with and without SPE procedure.