Table 2 Recovery of phytohormones from different sample matrices on polystyrene/ divinylbenzene-based Cromabond® HR-XC material
From: An UPLC-MS/MS method for highly sensitive high-throughput analysis of phytohormones in plant tissues
Matrix | SA | ABA | JA | 12-OH-JA | JA-Ile | OPDA | ||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Â | Slope | R 2 | Rec. | Slope | R 2 | Rec. | Slope | R 2 | Rec. | Slope | R 2 | Rec. | Slope | R 2 | Rec. | Slope | R 2 | Rec. |
Recovery control | 453.48 | 0.99 | 1.00 | 75.12 | 0.99 | 1.00 | 105.27 | 0.99 | 1.00 | 431.91 | 0.99 | 1.00 | 541.36 | 0.99 | 1.00 | 126.64 | 0.99 | 1.00 |
20% Methanol | 397.59 | 0.99 | 0.88 | 90.73 | 0.99 | 1.21 | 110.37 | 0.97 | 1.05 | 497.68 | 1.00 | 1.15 | 533.24 | 1.00 | 0.98 | 122.33 | 1.00 | 0.97 |
Methanolic extract | 558.56 | 0.96 | 1.23 | 76.50 | 0.92 | 1.02 | 108.49 | 0.97 | 1.03 | 444.99 | 0.98 | 1.03 | 532.82 | 1.00 | 0.98 | 118.41 | 0.99 | 0.93 |
Reconstituted methanolic extract | 392.81 | 0.77 | 0.86 | 55.25 | 0.86 | 0.49 | 81.27 | 0.88 | 0.64 | 156.24 | 0.83 | 0.44 | 424.25 | 0.99 | 0.67 | 77.65 | 0.95 | 0.50 |