Figure 3From: FACS-based purification of Arabidopsis microspores, sperm cells and vegetative nucleiSperm cell viability before and after sorting. Cell viability assays were performed by staining cells before and after FACS with SYTOX orange dye, which stains only dead cells. Sperm cells (SC) before and after sorting were confirmed to be viable only when prepared in Sperm Extraction Buffer (SEB), while in Galbraith’s buffer (NEB) we observed 100% staining.Back to article page