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Table 3 Percent recovery of internal standards after extraction and purification

From: Concurrent profiling of indole-3-acetic acid, abscisic acid, and cytokinins and structurally related purines by high-performance-liquid-chromatography tandem electrospray mass spectrometry

Internal standard

% Recovery from plant material

[ 2 H 5 ](OG)Z

70

[ 2 H 3 ](9G)Z

75

[ 2 H 5 ]Z

10

[ 2 H 5 ](OG)[9R]Z

97

[ 2 H 5 ][9R]Z

60

[ 2 H 5 ][9RNT]Z

10

[ 2 H 5 ](MeS)Z

11

[ 2 H 5 ](MeS)[9R]Z

78

[ 2 H 7 ](OG)DZ

102

[ 2 H 3 ]DZ

30

[ 2 H 7 ] (OG)[9R]DZ

47

[ 2 H 3 ][9RNT]DZ

10

[ 2 H 3 ][9R]DZ

83

[ 2 H 6 ](7G)iP

82

[ 2 H 6 ]iP

43

[ 2 H 6 ][9R]iP

28

[ 2 H 6 ][9RNT]iP

14

[ 2 H 6 ](MeS)[9R]iP

65

[ 2 H 6 ](MeS)iP

6

[ 2 H 7 ]BA

5

[ 2 H 7 ][9R]BA

63

[ 13 C 5 ] oT

10

[ 13 C 5 ]Ade

75

[ 13 C 5 ]Ado

90

U 15 N 5 Ino

60

U 15 N 5 AMP

25

[ 13 C 6 ]IAA

32

d 4 ABA

39

  1. Percent recovery was calculated using calibration curves.
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Plant Methods

ISSN: 1746-4811