Concurrent profiling of indole-3-acetic acid, abscisic acid, and cytokinins and structurally related purines by high-performance-liquid-chromatography tandem electrospray mass spectrometry

Farrow, Scott C; Emery, RJ Neil

doi:10.1186/1746-4811-8-42

Plant Methods

Table 2 Quantification of endogenous cytokinins, selected purines, abscisic acid and indole-3-acetic acid from Arabidopsis thaliana leaf

From: Concurrent profiling of indole-3-acetic acid, abscisic acid, and cytokinins and structurally related purines by high-performance-liquid-chromatography tandem electrospray mass spectrometry

Concentration (pmol^.g^{-1 .} FW^-1)
Metabolite	This Study (4-week old leaves, n=4)	[40] (10-day old Arabidopsis seedlings)	[65] (7 youngest leaves/various organs of Arabidopsis)	[66] (Arabidopsis leaves)	[67] (Arabidopsis plants)	[60] (tea leaves)	[17] (Pisum sativum fruits – old method)	[38] (various organs from Oryza sativa)
Z	4.67 ± 1.92	0.61	0-2	N.M.^a	N.M.	N.M.	0-25	0-3
c Z	6.08 ± 1.03	0.32	N.M.	N.M.	N.M.	N.M.	N.D.	0-12
DZ	N.D.^b	0.02	0-2.5	N.M.	N.M.	N.M.	0-10	N.M.
iP	7.65 ± 1.09	0.42	0-2	N.M.	N.M.	N.M.	0-10	0-6
[9R]Z	1.19 ± 0.48	2.84	2.5-20	N.M.	N.M.	N.M.	0-25	0-1
*(cis)* [9R]Z	2.42 ± 0.11	1.13	N.M.	N.M.	N.M.	N.M.	50-300	0-10
[9R]DZ	0.14 ± 0.07	0.09	0-2	N.M.	N.M.	N.M.	0-20	0-1
[9R]iP	1.95 ± 0.39	2.38	2-100	N.M.	N.M.	N.M.	10-100	0-5
[9RNT]Z	9.41-NTP^d ± 5.27	1.62-MP^e	4-45-NTP	N.M.	N.M.	N.M.	10-50-NTP	0-2.5
*(cis)* [9RNT]Z	7.42-NTP ± 0.62	N.M.	N.M.	N.M.	N.M.	N.M.	10-200	0-7
[9RNT]DZ	N.D.–NTP	N.M.	0-5-NTP	N.M.	N.M.	N.M.	10-50	N.M.
[9RNT]iP	13.5-NTP ± 5.24	5.69-MP	15-60-NTP	N.M.	N.M.	N.M.	2-350-NTP	0-19
(OG)[9R]Z	3.19 ± 0.31	0.52	N.M.	N.M.	N.M.	N.M.	N.M.	0-0.6
*(cis)* (OG)[9R]Z	3.43 ± 0.31	0.48	N.M.	N.M.	N.M.	N.M.	N.M.	0-50
(OG)[9R]DZ	1.31 ± 0.41	N.M.	N.M.	N.M.	N.M.	N.M.	N.M.	N.M.
(9G)Z	18.83 ± 2.5	3.72	15-20	N.M.	N.M.	N.M.	N.M.	0-160
(7G)iP	N.M.	N.M.	N.M.	N.M.	N.M.	N.M.	N.M.	0-0.2
IAA	749.76 ± 111	N.M.	N.M.	28-143	N.M.	N.M.	N.M.	10-300
ABA	101.58 ± 30	N.M.	N.M.	N.M.	22.7	N.M.	N.M.	70-1000
Ade	2039.73 ± 251	N.M.	N.M.	N.M.	N.M.	N.M.	N.M.	N.M.
Ado	1742.93 ± 188	N.M.	N.M.	N.M.	N.M.	N.M.	N.M.	N.M.
Ino	725.00 ± 343.5	N.M.	N.M.	N.M.	N.M.	N.M.	N.M.	N.M.
Amp	24988.84 ± 4702	N.M.	N.M.	N.M.	N.M.	15000-57000^c	N.M.	N.M.

We compared our values to our old method and with those from the literature to demonstrate the robustness and accuracy of our method. It should be noted that differences could be due to tissue type, growth stage, growth conditions, and not the analytical techniques.
^aN.M. = Not Monitored; ^bN.D. = Not Detected; ^c = expressed as (pmol^. g^-1. DW^-1); ^dNTP = nucleotide pool; ^eMP = CK-monophosphat.

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ISSN: 1746-4811

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