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Table 2 Sucrose-uptake by sycamore cells incubated in Pi-supplied and Pi-deprived NM at 22°C and 5°C

From: A simple and efficient method for the long-term preservation of plant cell suspension cultures

 

Pi-supplied NM

Pi- starved NM

Temperature

22°C

5°C

22°C

5°C

Sucrose uptake (mg d-1 g-1)

51 ± 5

9.7 ± 0.9

17 ± 1.5

3.4 ± 0.3

  1. Cells were initially incubated for 5 d at 22°C in either Pi-supplied or Pi-free Lamport's NM. At day 5, incubation was pursued at 22°C or 5°C and sucrose-uptake expressed as mg d-1 g-1 cell FW was measured as indicated in "Material and Methods". Values are means ± SD (n = 5).
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Plant Methods

ISSN: 1746-4811