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Table 1 O2-uptake by sycamore and Arabidopsis cells incubated at different temperatures

From: A simple and efficient method for the long-term preservation of plant cell suspension cultures

 

sycamore

 

Arabidopsis

  

Temperature

 

+ 2 μM FCCP

 

+ 2 μM FCCP

+ light

22°C

350 ± 25

550 ± 50

480 ± 40

780 ± 70

330 ± 30

5°C

72 ± 6

145 ± 12

105 ± 10

200 ± 15

30 ± 3

5°C→22°C

360 ± 30

560 ± 50

470 ± 40

750 ± 70

290 ± 25

  1. The O2-uptake by Sycamore and Arabidopsis cells harvested 5 d after subculture at 22°C and 20 d after subculture at 5°C was measured in their respective culture media at 22°C or 5°C. In addition, the O2-uptake by cells cultivated for 20 d at 5°C was measured at 22°C after a 10-min recovery (5°C→22°C). O2-uptake rates were measured in the dark, except for Arabidopsis cells when specified (+ light), as indicated in "Material and Methods". They are expressed as nmol O2 consumed min-1 g-1 cell FW. Values are means ± SD (n = 5). FCCP, cyanide p-trifluoromethoxyphenylhydrazone.
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Plant Methods

ISSN: 1746-4811