Figure 6
From: A simple and efficient method for the long-term preservation of plant cell suspension cultures
In vivo proton-decoupled 31P-NMR spectra showing the recovery of sycamore cells preserved over 6 months. Cells were preserved over 6 months at 5°C in a Pi-free nutrient medium. They were then placed in the perfusion system dedicated to in vivo NMR analyses as described in "Material and Methods". At time zero, 100 μM Pi was added to NM and the temperature was set to 22°C. Spectra are the result of 1500 transients with a 0.6-s repetition time (15 min). Peak assignments are as follows: ref, reference (methylenediphosphonate) used to measure chemical shifts and for quantification; glc-6-P, glucose 6-phosphate; fru-6-P, fructose 6-P; cyt-Pi, cytoplasmic-Pi; vac-Pi, vacuolar Pi; NTP, nucleoside triphosphate; UDP-glc, uridine-5'-diphosphate-α-D-glucose.