Figure 5
From: A simple and efficient method for the long-term preservation of plant cell suspension cultures
Growth of sycamore cells under different culture conditions. Growth of suspension-cultured sycamore cells was measured in a Pi-free nutrient medium at 22°C followed by cell preservation at 5°C, and recovery in a Pi-supplied medium at 22°C. Cells were cultivated as described in "Material and Methods". They originated from an exponentially growing cell suspension repeatedly subcultured in Pi-supplied NM before the growth plateau. At time zero, cells were incubated at 5.0 mg ml-1 in Pi-free Lamport's NM at 22°C. After a short period of exponential growth, a growth plateau corresponding to a cell concentration of only 20 mg cell FW ml-1 culture was attained due to Pi starvation. At that stage, the temperature was set to 5°C. Six months later preserved cells were incubated under standard culture conditions and subcultured (day 190). This experiment was repeated five times. In semi-logarithmic plot, standard deviation is of the order of the symbol size.