Figure 1
From: A simple and efficient method for the long-term preservation of plant cell suspension cultures
Growth of suspension-cultured sycamore cells at 22°C and 5°C. Cells were cultivated in Lamport's nutrient medium as described in "Material and Methods". They originated from an exponentially growing cell suspension maintained at 22°C and repeatedly subcultured before the growth plateau. The cell concentration (FW) at time zero was 5.0 mg ml-1 culture. At 22°C (a), cells grew exponentially for 10 d (linear on semi-logarithmic plot) before reaching a plateau. At day 14, the dilution of cell suspension to their initial concentration (subculture) restarted the exponential cell growth after a 2-d lag. At 5°C (b), cells grew exponentially for 40 d before reaching a plateau. In separate experiments (arrow in Figure 1b), the temperature was set back to 22°C after 20 d of culture at 5°C. This experiment was repeated five times. In semi-logarithmic plot, standard deviation is of the order of the symbol size.