Simple and efficient methods to generate split roots and grafted plants useful for long-distance signaling studies in Medicago truncatula and other small plants

Table 1 Success rate of shoot to root reciprocal grafting using a modification of the inverted-Y technique

Graft combination	Grafts initiated	Successful grafts	Success rate (%)
Wild type/Wild type	24	18	75
Mutant-1/Mutant-1	21	11	52.4
Mutant-2/Mutant-2	23	14	60.9
Mutant-1/Wild type	12	10	83.3
Mutant-1/Mutant-2	23	14	60.9
Mutant-2/Mutant-1	24	15	62.5
Mean/-SE	21.2/-1.9	13.7/-1.2	65.8/-4.6

The description of the graft combination refers to Wild type:ecotype Jemalong A17, mutant-1: the shoot controlled hypernodulator sunn-4[41] and mutant-2: the root controlled hypernodulator rdn1-2[30].

ISSN: 1746-4811