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Figure 4 | Plant Methods

Figure 4

From: Simple and efficient methods to generate split roots and grafted plants useful for long-distance signaling studies in Medicago truncatula and other small plants

Figure 4

Creating an inverted-Y graft between two different rootstock genotypes. (A) Schematic representation of inverted-Y grafting. Genotype A is the main stock which contributes both the shoot genotype and one of the rootstocks and genotype B is acting as the insert contributing only one of the rootstocks. (B) Close up of graft immediately after insertion. (D) Four day old plants immediately after grafting on BNM media covered with brown seed germination paper. (D and E) Successful grafts immediately before transferring to a Perlite system. Red arrows in C indicate the new root growth in the successful grafts while blue arrows indicate failed grafts that lack new growth. (F) Representative plant 21 days after the second root inoculation; washed and ready for nodule counting.

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Plant Methods

ISSN: 1746-4811