Skip to main content
Figure 2 | Plant Methods

Figure 2

From: Simple and efficient methods to generate split roots and grafted plants useful for long-distance signaling studies in Medicago truncatula and other small plants

Figure 2

Generating split roots in M. truncatula.(A) Plants on Petri dishes before cutting roots, (B) after cutting roots and (C) after placing filter paper over the roots. (D) Lateral root initiation 5 days after cutting the root. (E) Example of 2 plants trimmed to two balanced root systems ready for transfer to Perlite. (F) Plants with two root systems, one growing in each pot (G) Experimental design of pots in a standard greenhouse tray that maximizes plants per unit space. Each row is a replicate, and each column contains the following (a) Root A of plant 1 (b) Root B of plant 1 and Root B of plant 2 (c) Root A of plant 2 and Root A of plant 3 (d) Root B of plant 3 and Root B of plant 4 (e) Root A of plant 4 (H) A plant 21 days after Root B inoculation ready for counting.

Back to article page

Plant Methods

ISSN: 1746-4811