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Figure 4 | Plant Methods

Figure 4

From: Development of a rapid and inexpensive method to reveal natural antisense transcripts

Figure 4

Validation of the method through RT-PCR using gene-specific primers. Sense: RT-PCR carried out using OeSLG_full-length_5’-tag_REV and subsequent amplification using primer_TAG and OeSLG_internal_FOR. Antisense: RT-PCR carried out using OeSLG_full-length_5’-tag_FOR and subsequent amplification using primer_TAG and OeSLG_internal_REV. The sense OeSLG was expressed in both pistils and anthers, whereas the antisense OeSLG was expressed almost exclusively in anthers. OeEF: Elongation Factor α1 used as positive internal control.

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