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Figure 2 | Plant Methods

Figure 2

From: Barley callus: a model system for bioengineering of starch in cereals

Figure 2

Schematic workflow of the callus starch models system. The scheme presents the essential steps. (a) Immature embryo transformation. (b) Callus containing both transgenic and non-trangenic parts. Transgenic parts are identified by GFP, excised and sub-cultured (c) Fully transgenic callus is obtained. (d) Callus cultures maintenance with subculturing steps every three weeks. Sampling and starch analysis. (e) Transgenic plants can be regenerated.

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