Table 2 Comparison of methods to assess powdery mildew infection
From: Rapid quantification of plant-powdery mildew interactions by qPCR and conidiospore counts
Macroscopic categorization | Host cell entry counts | Conidiophore counts | Hyphal area quantification | qPCR | Conidia counts | |
|---|---|---|---|---|---|---|
Assay type | semi-quantitative | quantitative | quantitative | quantitative | quantitative | quantitative |
Staining required? | no | yes | yes | yes | no | no |
Microscopy required? | no | yes (hundreds of interaction sites) | yes (multiple colonies) | yes (multiple colonies) | no | yes |
Stage of patho-genesis scored | late (conidiation) | early (host cell entry) | late (conidiation) | early to late (host cell entry to conidiation) | middle to late (hyphal expansion to conidiation) | late (conidiation) |
Importance of equal inoculation density | high (no normalization) | low (internal normalization) | low (internal normalization) | low (internal normalization) | low (internal normalization) | medium (averaging effect of scoring multiple plants at once) |
Suitable for high-throughput analysis | yes | no | no | no | yes | yes |
Reference | this publication | this publication |