From: An improved allele-specific PCR primer design method for SNP marker analysis and its application
SNP types | mismatch types at 3’end of primer in 01-88 | Destabilization strength of mismatch type |
---|---|---|
(01-88/02-12) | ||
A/G | TG | Strong |
C/T | GT | |
G/A | AC | |
T/C | CA | |
A/C | TC | Weak |
G/T | CT | |
C/A | AG | |
T/G | GA | |
A/T | TT | Strong and Medium |
T/A | AA | |
G/C | CC | Strong and Medium |
C/G | GG |