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Table 2 Destabilization strength of eight combinations of mismatch nucleotide pairing

From: An improved allele-specific PCR primer design method for SNP marker analysis and its application

SNP types

mismatch types at 3’end of primer in 01-88

Destabilization strength of mismatch type

(01-88/02-12)

  

A/G

TG

Strong

C/T

GT

G/A

AC

T/C

CA

A/C

TC

Weak

G/T

CT

C/A

AG

T/G

GA

A/T

TT

Strong and Medium

T/A

AA

G/C

CC

Strong and Medium

C/G

GG