Figure 1
From: An improved allele-specific PCR primer design method for SNP marker analysis and its application
Schematic representation of the AS-PCR primer design. a, Primer P1 forms a perfect match with allele from 02-12, but a mismatch base pair at the 3’end with the DNA sequence of allele from 01-88. It could amplify the band in both of two lines 01-88 and 02-12. Primer P2 forms two mismatch base pairs with allele from 01-88 at the 3’end and in the 3rd nucleotide from the 3’end, while a mismatch base pair in the 3rd nucleotide with allele from 02-12. It amplified the band only in 02-12. b, Schematic representation of different mismatches during the SNP primer design.