From: Universal Multiplex PCR: a novel method of simultaneous amplification of multiple DNA fragments
Name | Sequence (5' to 3') | Tm (°C) | References |
---|---|---|---|
universal adapter-F | CTCGTAGACTGCGTACCA | 57.30 | This study |
universal adapter-R | TACTCAGGACTCATCGTC | 55.02 | This study |
common adapter | CCTTCCTTCCTTCCCCCC | 61.86 | Bai et al., 2009 |
phi085-F | AGCAGAACGGCAAGGGCTACT | 61.92 | MaizeGDB |
phi085-R | TTTGGCACACCACGACGA | 57.30 | MaizeGDB |
U-phi085-F | CTCGTAGACTGCGTACCA AGCAGAACGGCAAGGGCTACT | 72.61 | This study |
U-phi085-R | TACTCAGGACTCATCGTC TTTGGCACACCACGACGA | 70.05 | This study |
C-phi085-F | CCTTCCTTCCTTCCCCCC AGCAGAACGGCAAGGGCTACT | 74.71 | This study |
C-phi085-R | CCTTCCTTCCTTCCCCCC TTTGGCACACCACGACGA | 73.47 | This study |
phi041-F | TTGGCTCCCAGCGCCGCAAA | 63.95 | MaizeGDB |
phi041-R | GATCCAGAGCGATTTGACGGCA | 61.94 | MaizeGDB |
U-phi041-F | CTCGTAGACTGCGTACCA TTGGCTCCCAGCGCCGCAAA | 73.96 | This study |
U-phi041-R | TACTCAGGACTCATCGTC GATCCAGAGCGATTTGACGGCA | 71.33 | This study |
C-phi041-F | CCTTCCTTCCTTCCCCCC TTGGCTCCCAGCGCCGCAAA | 76.12 | This study |
C-phi041-R | CCTTCCTTCCTTCCCCCC GATCCAGAGCGATTTGACGGCA | 74.40 | This study |
phi123-F | GGAGACGAGGTGCTACTTCTTCAA | 61.97 | MaizeGDB |
phi123-R | TGTGGCTGAGGCTAGGAATCTC | 61.94 | MaizeGDB |
U-phi123-F | CTCGTAGACTGCGTACCA GGAGACGAGGTGCTACTTCTTCAA | 71.87 | This study |
U-phi123-R | TACTCAGGACTCATCGTC TGTGGCTGAGGCTAGGAATCTC | 71.33 | This study |
C-phi123-F | CCTTCCTTCCTTCCCCCC GGAGACGAGGTGCTACTTCTTCAA | 73.82 | This study |
C-phi123-R | CCTTCCTTCCTTCCCCCC TGTGGCTGAGGCTAGGAATCTC | 74.40 | This study |
umc1478-F | GAAGCTTCTCCTCTCGCGTCTC | 63.80 | MaizeGDB |
umc1478-R | CAGTCCCAGACCCTAGCTCAGTC | 65.52 | MaizeGDB |
U-umc1478-F | CTCGTAGACTGCGTACCA GAAGCTTCTCCTCTCGCGTCTC | 73.38 | This study |
U-umc1478-R | TACTCAGGACTCATCGTC CAGTCCCAGACCCTAGCTCAGTC | 73.10 | This study |
C-umc1478-F | CCTTCCTTCCTTCCCCCC GAAGCTTCTCCTCTCGCGTCTC | 75.43 | This study |
C-umc1478-R | CCTTCCTTCCTTCCCCCC CAGTCCCAGACCCTAGCTCAGTC | 76.10 | This study |
umc1268-F | ACGAACAACCTAGCACAGTCCTAAA | 60.34 | MaizeGDB |
umc1268-R | CAAGGCGGTTACCAAGTTTACATC | 60.26 | MaizeGDB |
U-umc1268-F | CTCGTAGACTGCGTACCA ACGAACAACCTAGCACAGTCCTAAA | 70.70 | This study |
U-umc1268-R | TACTCAGGACTCATCGTC CAAGGCGGTTACCAAGTTTACATC | 69.92 | This study |
C-umc1268-F | CCTTCCTTCCTTCCCCCC ACGAACAACCTAGCACAGTCCTAAA | 71.93 | This study |
C-umc1268-R | CCTTCCTTCCTTCCCCCC CAAGGCGGTTACCAAGTTTACATC | 72.85 | This study |
phi120-F | TGATGTCCCAGCTCTGAACTGAC | 61.92 | MaizeGDB |
phi120-R | GACTCTCACGGCGAGGTATGA | 61.95 | MaizeGDB |
U-phi120-F | CTCGTAGACTGCGTACCA TGATGTCCCAGCTCTGAACTGAC | 72.10 | This study |
U-phi120-R | TACTCAGGACTCATCGTC GACTCTCACGGCGAGGTATGA | 71.56 | This study |