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Table 1 Sequences of adapters and primers

From: Universal Multiplex PCR: a novel method of simultaneous amplification of multiple DNA fragments

Name

Sequence (5' to 3')

Tm (°C)

References

universal adapter-F

CTCGTAGACTGCGTACCA

57.30

This study

universal adapter-R

TACTCAGGACTCATCGTC

55.02

This study

common adapter

CCTTCCTTCCTTCCCCCC

61.86

Bai et al., 2009

phi085-F

AGCAGAACGGCAAGGGCTACT

61.92

MaizeGDB

phi085-R

TTTGGCACACCACGACGA

57.30

MaizeGDB

U-phi085-F

CTCGTAGACTGCGTACCA AGCAGAACGGCAAGGGCTACT

72.61

This study

U-phi085-R

TACTCAGGACTCATCGTC TTTGGCACACCACGACGA

70.05

This study

C-phi085-F

CCTTCCTTCCTTCCCCCC AGCAGAACGGCAAGGGCTACT

74.71

This study

C-phi085-R

CCTTCCTTCCTTCCCCCC TTTGGCACACCACGACGA

73.47

This study

phi041-F

TTGGCTCCCAGCGCCGCAAA

63.95

MaizeGDB

phi041-R

GATCCAGAGCGATTTGACGGCA

61.94

MaizeGDB

U-phi041-F

CTCGTAGACTGCGTACCA TTGGCTCCCAGCGCCGCAAA

73.96

This study

U-phi041-R

TACTCAGGACTCATCGTC GATCCAGAGCGATTTGACGGCA

71.33

This study

C-phi041-F

CCTTCCTTCCTTCCCCCC TTGGCTCCCAGCGCCGCAAA

76.12

This study

C-phi041-R

CCTTCCTTCCTTCCCCCC GATCCAGAGCGATTTGACGGCA

74.40

This study

phi123-F

GGAGACGAGGTGCTACTTCTTCAA

61.97

MaizeGDB

phi123-R

TGTGGCTGAGGCTAGGAATCTC

61.94

MaizeGDB

U-phi123-F

CTCGTAGACTGCGTACCA GGAGACGAGGTGCTACTTCTTCAA

71.87

This study

U-phi123-R

TACTCAGGACTCATCGTC TGTGGCTGAGGCTAGGAATCTC

71.33

This study

C-phi123-F

CCTTCCTTCCTTCCCCCC GGAGACGAGGTGCTACTTCTTCAA

73.82

This study

C-phi123-R

CCTTCCTTCCTTCCCCCC TGTGGCTGAGGCTAGGAATCTC

74.40

This study

umc1478-F

GAAGCTTCTCCTCTCGCGTCTC

63.80

MaizeGDB

umc1478-R

CAGTCCCAGACCCTAGCTCAGTC

65.52

MaizeGDB

U-umc1478-F

CTCGTAGACTGCGTACCA GAAGCTTCTCCTCTCGCGTCTC

73.38

This study

U-umc1478-R

TACTCAGGACTCATCGTC CAGTCCCAGACCCTAGCTCAGTC

73.10

This study

C-umc1478-F

CCTTCCTTCCTTCCCCCC GAAGCTTCTCCTCTCGCGTCTC

75.43

This study

C-umc1478-R

CCTTCCTTCCTTCCCCCC CAGTCCCAGACCCTAGCTCAGTC

76.10

This study

umc1268-F

ACGAACAACCTAGCACAGTCCTAAA

60.34

MaizeGDB

umc1268-R

CAAGGCGGTTACCAAGTTTACATC

60.26

MaizeGDB

U-umc1268-F

CTCGTAGACTGCGTACCA ACGAACAACCTAGCACAGTCCTAAA

70.70

This study

U-umc1268-R

TACTCAGGACTCATCGTC CAAGGCGGTTACCAAGTTTACATC

69.92

This study

C-umc1268-F

CCTTCCTTCCTTCCCCCC ACGAACAACCTAGCACAGTCCTAAA

71.93

This study

C-umc1268-R

CCTTCCTTCCTTCCCCCC CAAGGCGGTTACCAAGTTTACATC

72.85

This study

phi120-F

TGATGTCCCAGCTCTGAACTGAC

61.92

MaizeGDB

phi120-R

GACTCTCACGGCGAGGTATGA

61.95

MaizeGDB

U-phi120-F

CTCGTAGACTGCGTACCA TGATGTCCCAGCTCTGAACTGAC

72.10

This study

U-phi120-R

TACTCAGGACTCATCGTC GACTCTCACGGCGAGGTATGA

71.56

This study

  1. Tms are taken from the primer synthesis report card.