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Figure 6 | Plant Methods

Figure 6

From: Protocol: High-throughput and quantitative assays of auxin and auxin precursors from minute tissue samples

Figure 6

Examples of GC-MS results from typical plant extracts. (A) Chromatographic result from quantification of IPyA in 9.5 mg of Arabidopsis seedling tissue using GC-SRM-MS. (B) Measurements of plant endogenous IPyA, IAA, and IBA using different plant tissues, and analysis of IAA biosynthesis 4 hr after incubating tomato shoots with 0.1 mM [13C1]indole. One-week-old Arabidopsis seedlings and 5-day-old tomato seedlings were used for the studies. Average values of four replicates and standard errors are shown.

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