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Figure 4 | Plant Methods

Figure 4

From: Methods of staining and visualization of sphingolipid enriched and non-enriched plasma membrane regions of Arabidopsis thaliana with fluorescent dyes and lipid analogues

Figure 4

Time-dependent polarization of protoplasts with Laurdan. Time-dependent polarization of protoplasts as indicated by Laurdan; the more red shifted emission spectrum of Laurdan (A; F; K) indicated polar phases of higher water content, whereas a more blue shifted emission spectrum (here: green, false color; B; G; L) indicated apolar phases harboring less water; (C; H; M): merged images; (E; J; O): GP-values, corresponding to the GP-scale (−1: pure water phase; +1: fully ordered phase). (A-D) in freshly isolated protoplasts there was no polarization detectable, GP-values ranged from −0.3 to 0.2 (E); (F-J) 15 h post cell wall removal a lipid polarization was detected; at the lateral sides of the membrane areas of high lipid order emerged (J, arrows); (K-N) 20 h post cell wall removal a wide part of the plasma membrane appeared as polarized (O, arrow), accompanied by GP-values of up to 0.8.

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Plant Methods

ISSN: 1746-4811