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Figure 3 | Plant Methods

Figure 3

From: Methods of staining and visualization of sphingolipid enriched and non-enriched plasma membrane regions of Arabidopsis thaliana with fluorescent dyes and lipid analogues

Figure 3

BD-SM at the plasma membrane and in the DRM-fraction. BD-SM appearance at the plasma membrane and in the DRM-fraction. (A &B) BD-SM reliably stained plasma membranes in protoplasts without losing cell viability. Fluorescence (A) and transmission image of (B) a BD-SM stained protoplast. BD-SM fluorescence was specific to the plane of the membrane (C &D). When applied to purified plasma membranes BD-SM appeared in the Arabidopsis DRM-fraction. Fluorescence (C) and transmission image (D) of purified membranes from the DRM-fraction. (E) is showing a bunch of protoplasts after trypan blue treatment; trypan blue is excluded from the cytosol of intact cells (here shown after FM4-64 treatment; in protoplasts treated with LRB-PE, DiIC12, DiIC18, DiD, BD-SM and Laurdan similar results were obtained using trypan blue).

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Plant Methods

ISSN: 1746-4811