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Figure 2 | Plant Methods

Figure 2

From: Methods of staining and visualization of sphingolipid enriched and non-enriched plasma membrane regions of Arabidopsis thaliana with fluorescent dyes and lipid analogues

Figure 2

Single dye staining of protoplasts. Single dye staining of protoplasts, using FM4-64 (A; C), LRB-PE (E), DiIC12 (G), DiIC18 (I), and DiD (K); all dyes appeared at the plasma membrane. Upon FM4-64 treatment first fluorescent vesicles appeared in the cytoplasm within 20 minutes; endocytosis events were strongly enhanced with ongoing incubation time (C; 30 minutes post FM4-64 incubation). All staining protocols were designed with respect to cell viability; macroscopically the protoplasts were not affected and maintained their round shape (B; D; F; H; J; L). Viability was successfully tested using trypan blue.

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