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Figure 5 | Plant Methods

Figure 5

From: Method: low-cost delivery of the cotton leaf crumple virus-induced gene silencing system

Figure 5

 ChlI  silencing from two different VIGS vectors inoculated using two low-cost particle inflow guns. A) ChlI silencing in cotton (64 dpi) co-bombarded with the CLCrVA:ChlI and CLCrVB plasmids using the modified plastic water gun shown in Figure 4A,B. B) N. benthamiana (37 dpi) showing the viral symptom of contorted upper leaves (arrows) after bombardment with the wild type TGMV virus using the homemade metal gene gun. C) N. benthamiana (37 dpi) showing ChlI silencing after co-bombardment with TGMVA and TGMVB:ChlI using the metal gene gun (Figure 4C).

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