Figure 1From: Method: low-cost delivery of the cotton leaf crumple virus-induced gene silencing systemDetails of the CLCrV-derived VIGS vectors described in this paper. A, B) Maps of CLCrV plasmids for biolistic inoculation. C,D) Maps of the CLCrV:CP- (C) and CLCrVB (D) binary vectors for agroinoculation. The pBluescript SKII + (A,B) and the pCambia1300 (C,D) plasmid backbones are shown by a thin black line and geminivirus DNA is blue or red. Unique Sac I and Xba I sites that may be used to sub-clone silencing fragments from the biolistic vector into the Agrobacterium vector are marked with asterisks, and the resulting fragment is shown in red. MCS – multiple cloning site (light blue), CR – common region (green), LB and RB – left and right T-DNA borders respectively, HygR – hygromycin resistance, AmpR – ampicillin resistance, KanR – kanamycin resistance. AL1, AL2, AL3, and AL4 are genes encoded by the A-DNA and BL1 and BR1 by the B-DNA of CLCrV.Back to article page