Restriction digested and undigested sorghum leaf DNA. Five μg leaf DNA was digested in 20 μl reaction volume for 2 or 12 hours with 5 units of Bam HI (B), Eco RI (E), or Hind III (H). About 5 μl digestion was loaded each lane. UN, undigested leaf DNA, λ, phage λ DNA marker cut with Eco RI and Hind III. Similar digestion kinetics was observed in DNA extracted from dry seeds.