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Figure 3 | Plant Methods

Figure 3

From: Screening for in planta protein-protein interactions combining bimolecular fluorescence complementation with flow cytometry

Figure 3

Screening controls for the YN-cDNA and YC-cDNA libraries. X-axis is YFP fluorescence and Y-axis is autofluorescense; arrows show and indicate positive mYFP-BiFC signals. A. Mock transfected cells. B. Cells transfected with YN-Library alone. C. Cells transfected with free YN fragment alone. D. Cells co-transfected with YN-Library and free YC fragment. E. Cells co-transfected with bZIP63-YC and YN-Library; a clear YFP-BiFC signal was detected. F. Cells co-transfected with mYFP-YC and YN-Library; a clear YFP-BiFC signal was detected. G. Mock transfected cells. H. Cells transfected with the YC-Library alone. I. Cells co-transfected with mRFP-YN and YC-Library; only mRFP signal was detectable. J. Cells co-transfected with mCherry-YN173 and YC-Library; only mCherry signal was detectable. K. Cells co-transfected with bZIP63-YN and YC-Library. L. Cells co-transfected with CPK3-YN173 and YC-Library; a clear YFP-BiFC signal was detected.

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Plant Methods

ISSN: 1746-4811