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Figure 1 | Plant Methods

Figure 1

From: Screening for in planta protein-protein interactions combining bimolecular fluorescence complementation with flow cytometry

Figure 1

Design for performing in vivo BiFC screens by DNA transfection in protoplasts. The screen design is explained in detail in the text. In brief, DNA from a random library and specific bait are co-transfected into protoplasts. Protoplasts providing positive BiFC signals are identified and sorted by FACS. The DNA is harvested and the bacterial plasmids are recovered. The process is repeated with the new prey pool until a suitable number of putative bacterial colonies are obtained for further processing.

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