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Table 1 Key vectors used in this study

From: Robust reconstitution of active cell-cycle control complexes from co-expressed proteins in bacteria

Vector properties
Vector Gene 1 Gene 2 Cloning sites (Gene1) Cloning sites (Gene2) Replicon Antibiotic resistance
pHGGWA HisGST- CYCD3;1   Gateway   ColE1 Ampicillin
pHMGWA HisMBP-SDS   Gateway   ColE1 Ampicillin
  HisMBP-CYCB1;2   Gateway   ColE1 Ampicillin
pHGGWA HisGST- Wee1   Gateway   ColE1 Ampicillin
  HisGST- CDKF;1   Gateway   ColE1 Ampicillin
pCDFDuet-1 StrepIII- CDKA;1 GST-Cak1 Nco I - Not I Nde I - Xho I CloDF13 Spectinomycin
  StrepIII- CDKB1;1 GST-Cak1 Nco I - Not I Nde I - Xho I CloDF13 Spectinomycin
  StrepIII- CDKB2;2 GST-Cak1 Nco I - Not I (SLIC) Nde I - Xho I CloDF13 Spectinomycin
   GST-Cak1   Nde I - Xho I CloDF13 Spectinomycin
  StrepIII- CDKA;1   Nco I - Not I   CloDF13 Spectinomycin
   GST-CDKF;1   Nde I - Xho I CloDF13 Spectinomycin
  StrepIII- CDKA;1 GST-CDKF;1 NcoI - NotI Nde I - Xho I CloDF13 Spectinomycin
  1. sequence and ligation-independent cloning.