Table 1 Key vectors used in this study
From: Robust reconstitution of active cell-cycle control complexes from co-expressed proteins in bacteria
Vector properties | ||||||
|---|---|---|---|---|---|---|
Vector | Gene 1 | Gene 2 | Cloning sites (Gene1) | Cloning sites (Gene2) | Replicon | Antibiotic resistance |
pHGGWA | HisGST- CYCD3;1 | Â | Gateway | Â | ColE1 | Ampicillin |
pHMGWA | HisMBP-SDS | Â | Gateway | Â | ColE1 | Ampicillin |
| Â | HisMBP-CYCB1;2 | Â | Gateway | Â | ColE1 | Ampicillin |
pHGGWA | HisGST- Wee1 | Â | Gateway | Â | ColE1 | Ampicillin |
| Â | HisGST- CDKF;1 | Â | Gateway | Â | ColE1 | Ampicillin |
pCDFDuet-1 | StrepIII- CDKA;1 | GST-Cak1 | Nco I - Not I | Nde I - Xho I | CloDF13 | Spectinomycin |
| Â | StrepIII- CDKB1;1 | GST-Cak1 | Nco I - Not I | Nde I - Xho I | CloDF13 | Spectinomycin |
|  | StrepIII- CDKB2;2 | GST-Cak1 | Nco I - Not I (SLIC†) | Nde I - Xho I | CloDF13 | Spectinomycin |
| Â | Â | GST-Cak1 | Â | Nde I - Xho I | CloDF13 | Spectinomycin |
| Â | StrepIII- CDKA;1 | Â | Nco I - Not I | Â | CloDF13 | Spectinomycin |
| Â | Â | GST-CDKF;1 | Â | Nde I - Xho I | CloDF13 | Spectinomycin |
| Â | StrepIII- CDKA;1 | GST-CDKF;1 | NcoI - NotI | Nde I - Xho I | CloDF13 | Spectinomycin |