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Figure 8 | Plant Methods

Figure 8

From: Use of ex vitro composite plants to study the interaction of cowpea (Vigna unguiculata L.) with the root parasitic angiosperm Striga gesnerioides

Figure 8

qRT-PCR analysis  RSG3-301  transcript levels in transgenic and non-transgenic roots of cowpea composite plants. Transcripts encoding the RSG3-301 resistance gene were determined by qRT-PCR using total RNA isolated from transgenic and non-transgenic roots of composite Blackeye plants generated using the following constructs: pK7WG2D (prolD-GFP, no RSG3-301 gene), pK7WG2 (no GFP, no RSG3-301 gene), pKMG-RSG3-301 (prolD-GFP, overexpressed RSG3-301 gene) and pKM0-RSG-301 (no GFP, overexpressed RSG3-301 gene). Relative RSG3-301 transcript levels were obtained from calibrating its threshold cycles relative to control actin transcripts as described in the Materials and Methods. Means and standard errors (SE) based on from the 5 independent biological replicates are given.

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Plant Methods

ISSN: 1746-4811