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Figure 7 | Plant Methods

Figure 7

From: Use of ex vitro composite plants to study the interaction of cowpea (Vigna unguiculata L.) with the root parasitic angiosperm Striga gesnerioides

Figure 7

Quantitative analysis of resistance response of transgenic and non-transgenic roots on composite cowpea plants to attempted parasitism by  S. gesnerioides  race SG3. The number of parasite attachments showing either HR responses or tubercle growth were evaluated on the roots of composite cowpea plants generated by transformation with either pK7WG2D (which contains a prolD-GFP construct but lacks the RSG3-301 resistance gene) or pKMG-RSG3-301 (which contains a prolD-GFP construct and a CaMV35S-RSG3-301 resistance gene construct). The number of events was counted 8 dpi with S. gesnerioides race SG3. Data show the results of counts from 14 independent plants. Error bars indicate the standard error (SE). Events were counted on roots determined visually to be both transgenic and expressing GFP or non-transgenic and non-GFP fluorescent. Statistical significance was determined using one-way ANOVA. P < 0.001 is denoted by an asterisk.

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Plant Methods

ISSN: 1746-4811