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Figure 5 | Plant Methods

Figure 5

From: Use of ex vitro composite plants to study the interaction of cowpea (Vigna unguiculata L.) with the root parasitic angiosperm Striga gesnerioides

Figure 5

Analysis of GFP transcript levels in roots of composite cowpea plants. Transcript encoding GFP were measured in the roots of control Blackeye plants and in composite plants transformed with either pKM0-RSG-301 (vector with RSG3-301 minus GFP coding region), pK7WG2D (containing the prolD-GFP chimeric gene) and pCambia1300-Gmubi3 (containing the Gmubi3-GFP chimeric gene). PCR was carried out using gene specific primers directed to the GFP coding region. The predicted GFP amplification product is indicated; actin gene expression was used as control. The (+) indicates root tissues that gave a visual GFP fluorescence signal; (−) indicate root tissues that did not give a visual fluorescence signal.

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Plant Methods

ISSN: 1746-4811