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Figure 3 | Plant Methods

Figure 3

From: Use of ex vitro composite plants to study the interaction of cowpea (Vigna unguiculata L.) with the root parasitic angiosperm Striga gesnerioides

Figure 3

Factors affecting transformation efficiency and recovery of composite plants. A. Effects of multiplicity of infection during co-cultivation on the recovery of transgenic plants (transformation efficiency). B. Effects of location of root excision on transformation efficiency. Roots were cut at varied lengths (cm) below the cotyledon. C. Effect of temperature on transformation efficiency. Cowpea plants were co-cultivated with A. rhizogenes R1000 at the temperatures indicated. Number of transgenic roots was determined by presence of a GFP signal. Experiments were carried out in duplicate using up to 170 plants total. Values are means ± SE. Statistical significance was determined using one-way ANOVA. P < 0.05 is denoted by an asterisk. D. Percentage of newly formed transgenic roots on the positively transformed cowpea plant co-cultivated with A. rhizogenes at three different temperatures. Roots were assessed for GFP after emerging from a rockwool cube 12–14 days after transformation. The data show results from more than 1,700 roots total. Values are means ± SE. Statistical significance was determined using one-way ANOVA. P < 0.001 is denoted by an asterisk.

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Plant Methods

ISSN: 1746-4811