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Figure 2 | Plant Methods

Figure 2

From: Use of ex vitro composite plants to study the interaction of cowpea (Vigna unguiculata L.) with the root parasitic angiosperm Striga gesnerioides

Figure 2

Illustrations depicting the main steps for the production of  ex vitro  composite cowpea plants. A. Pregerminated seeds are placed between two pieces of rockwool for further development. B. A 10 day old cowpea plant growing in vitro in rockwool growth chamber prior to Agrobacterium rhizogenes infection. C. Cowpea with a cut off root inserted into a rockwool cube saturated with Agrobacterium rhizogenes harboring pK7WG2D with GFP. D. Stressing infected cowpea by drying until leaf wilting. E. Transgenic roots growing out of the rockwool cube 12–14 days after infection with A. rhizogenes. F. Cowpea plant with transformed hairy roots in a Petri dish rhizotron prior to Striga infection.

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