Figure 2
From: A new method to identify flanking sequence tags in chlamydomonas using 3’-RACE
3’-RACE amplification of flanking DNA. Strains are indicated at the top. Regular Phusion polymerase was used to amplify the CrAadA marker (left) or the control PETC transcript (right). Bottom panel: PCR1; top: PCR2. The New England Biolabs size ladders are indicated by arrowheads (black: 100 bp ladder; grey: 1 kb ladder). Black stars point to background bands found in negative controls, white stars to mutant–specific bands that eventually yielded FSTs. For each strain, the left lane shows samples where PCR used annealing at 60 °C, while the right lane used « touch down » from 72 to 60 °C in 12 cycles. Note that the latter yields less background and in general stronger specific bands.