Figure 1
From: A new method to identify flanking sequence tags in chlamydomonas using 3’-RACE
Principle of FST retrieval by 3’-RACE. A resistance cassette with a truncated 3’UTR is generated by PCR (red arrows). After its random integration in the genome, transcription from its promoter generates a chimeric mRNA containing the marker (CDS in cyan, UTRs in red) and the flanking Chlamydomonas DNA (blue). It is reverse-transcribed using a primer (green) that anneals to its poly-A tail. The first-strand cDNA thus obtained is amplified using a marker-gene specific primer GSP1 and primer B annealing to the end of the extension. A second amplification using nested primers GSP2 and A generates a specific product that is then sequenced using a third marker-specific primer.