Table 1 Optimized MS/MS conditions for quantifying phytohormones and metabolites
Analytea | Scan modeb | Precursor ion (m/z) Q1 | Product ion (m/z) Q3 | Declustering potential (V)c | Entrance potential (V)c | Collision energy (V)c | Collision cell exit potential (V)c |
|---|---|---|---|---|---|---|---|
SA | - | 136.9 | 92.9 | -47 | -6 | -22 | -3 |
NAAa | - | 185 | 141.0 | -25 | -3 | -12 | -7 |
ABA | - | 263 | 153.0 | -60 | -5 | -15 | -8 |
2H6ABAa | - | 269 | 159.1 | -57 | -5 | -16 | -8 |
JA | - | 209 | 59.0 | -60 | -6 | -24 | -8.6 |
DHJAa | - | 211 | 59.0 | -61 | -9 | -25 | -8 |
JA-Ile | - | 322.2 | 130.0 | -67 | -7.5 | -32 | -7 |
MeJA | + | 225.2 | 151.2 | 52 | 4 | 16 | 8 |
IAA | - | 173.9 | 129.9 | -53 | -4 | -13 | -15 |
IAA-Asp | - | 289 | 131.9 | -66 | -4 | -25 | -6 |
D2-IAAa | - | 176 | 131.9 | -48 | -4 | -14 | -6 |
Naringenin | - | 271.2 | 151.0 | -76 | -10 | -25 | -8 |
Sakuranetin | - | 285.1 | 119.0 | -85 | -10 | -44 | -5 |
Momilactone A | + | 315.2 | 271.3 | 82 | 4 | 18 | 6 |