Procedure in FSTVAL. FSTVAL involves two consecutive steps: 1.) validate FSTs and 2.) find the best mapping position to the genome. Users can input FSTs in FASTA, FASTQ, or PHD format. First, the regions of the border of T-DNA insertion, the adaptor for PCR amplification, and the binary vector are masked from FSTs, and the FSTs are validated. Next, FSTs are matched to the genome and categorized. The highest scoring region is selected as an FST integration site.