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Table 5 Cytokinin levels in Arabidopsis thaliana extracts determined by UHPLC-ESI(+)MS/MS

From: A new approach for cytokinin isolation from Arabidopsis tissues using miniaturized purification: pipette tip solid-phase extraction

CKs Cytokinin content (pmol g–1FW)
  Seedlings Shoots Roots Root segments
t Z n.d. n.d. 0.96 ± 0.12 2.60 ± 0.97
t ZR 2.23 ± 0.37 0.88 ± 0.32 3.27 ± 0.52 4.92 ± 0.94
t Z7G 37.99 ± 2.81 23.49 ± 3.07 6.54 ± 0.75 n.d.
t Z9G 3.88 ± 1.22 4.32 ± 1.31 2.08 ± 0.52 n.d.
t ZOG 9.25 ± 2.77 9.42 ± 1.75 7.10 ± 1.33 7.63 ± 2.45
t ZMP n.d. n.d. n.d. 8.04 ± 2.44
cZ n.d. n.d. n.d. 0.95 ± 0.33
c ZR 0.80 ± 0.16 1.14 ± 0.37 3.71 ± 0.79 2.28 ± 0.41
c Z9G n.d. n.d. 2.02 ± 0.47 n.d.
c ZOG 0.90 ± 0.27 1.04 ± 0.33 2.89 ± 0.66 3.35 ± 0.39
DHZR 0.64 ± 0.19 0.94 ± 0.27 0.97 ± 0.28 n.d.
DHZ7G 5.48 ± 1.28 5.78 ± 1.51 2.07 ± 0.42 n.d.
DHZ9G n.d. 0.71 ± 0.22 0.29 ± 0.07 n.d.
DHZOG 0.46 ± 0.13 0.28 ± 0.07 0.15 ± 0.05 n.d.
iP 0.24 ± 0.10 0.15 ± 0.04 0.56 ± 0.17 0.42 ± 0.14
iPR 1.96 ± 0.28 1.24 ± 0.26 2.18 ± 0.46 5.15 ± 0.60
iP7G 53.87 ± 2.86 65.76 ± 12.47 30.16 ± 4.57 n.d.
iPMP n.d. n.d. n.d. 13.14 ± 0.35
  1. Values are meansf ± SD (n = 12); n.d. – not detected.
  2. 10-day-old A. thaliana seedlings, roots and shoots and apical part of the root of 8-day-old plants (approximately 0.5 cm) were extracted in Bieleski buffer, purified by multi-StageTip microcolumns and measured by UHPLC-ESI(+)-MS/MS method.