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Table 5 Cytokinin levels in Arabidopsis thaliana extracts determined by UHPLC-ESI(+)MS/MS

From: A new approach for cytokinin isolation from Arabidopsis tissues using miniaturized purification: pipette tip solid-phase extraction

CKs

Cytokinin content (pmol g–1FW)

 

Seedlings

Shoots

Roots

Root segments

t Z

n.d.

n.d.

0.96 ± 0.12

2.60 ± 0.97

t ZR

2.23 ± 0.37

0.88 ± 0.32

3.27 ± 0.52

4.92 ± 0.94

t Z7G

37.99 ± 2.81

23.49 ± 3.07

6.54 ± 0.75

n.d.

t Z9G

3.88 ± 1.22

4.32 ± 1.31

2.08 ± 0.52

n.d.

t ZOG

9.25 ± 2.77

9.42 ± 1.75

7.10 ± 1.33

7.63 ± 2.45

t ZMP

n.d.

n.d.

n.d.

8.04 ± 2.44

cZ

n.d.

n.d.

n.d.

0.95 ± 0.33

c ZR

0.80 ± 0.16

1.14 ± 0.37

3.71 ± 0.79

2.28 ± 0.41

c Z9G

n.d.

n.d.

2.02 ± 0.47

n.d.

c ZOG

0.90 ± 0.27

1.04 ± 0.33

2.89 ± 0.66

3.35 ± 0.39

DHZR

0.64 ± 0.19

0.94 ± 0.27

0.97 ± 0.28

n.d.

DHZ7G

5.48 ± 1.28

5.78 ± 1.51

2.07 ± 0.42

n.d.

DHZ9G

n.d.

0.71 ± 0.22

0.29 ± 0.07

n.d.

DHZOG

0.46 ± 0.13

0.28 ± 0.07

0.15 ± 0.05

n.d.

iP

0.24 ± 0.10

0.15 ± 0.04

0.56 ± 0.17

0.42 ± 0.14

iPR

1.96 ± 0.28

1.24 ± 0.26

2.18 ± 0.46

5.15 ± 0.60

iP7G

53.87 ± 2.86

65.76 ± 12.47

30.16 ± 4.57

n.d.

iPMP

n.d.

n.d.

n.d.

13.14 ± 0.35

  1. Values are meansf ± SD (n = 12); n.d. – not detected.
  2. 10-day-old A. thaliana seedlings, roots and shoots and apical part of the root of 8-day-old plants (approximately 0.5 cm) were extracted in Bieleski buffer, purified by multi-StageTip microcolumns and measured by UHPLC-ESI(+)-MS/MS method.